urease activity protection with edta against nanoparticles (fe2o3 and fe3o4) inactivation

نویسندگان

s. sadeghi- kaji

b. shareghi

m. salavati

چکیده

in this study the effects of fe2o3 and fe3o4 magnetic nanoparticles and edta on urease activity was investigated. the effect of nano-fe2o3 and nano-fe3o4 on urease activity were investigated. urease activity was studies by uv-vis spectrophotometry at 40 °c at ph = 7.2 using sodium phosphate as buffer. measurements were carried out using 0.075 mg/ml of urease and a range of nano-fe2o3 and nano-fe3o4 concentrations between 0.002-0.006 mg/ml. it was found that by increasing the concentration of nano-fe2o3 and nano-fe3o4, urease activity will be decreased. on the other hand, nano-fe2o3 and nano-fe3o4 act as non competitive inhibitor for urease. urease protection studies were corried out by using different concentration of edta (0.004-0.008 mg/ml). it was shown by increasing the concentration of edta, the activity of enzyme increased.

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عنوان ژورنال:
journal of nanostructures

ناشر: university of kashan

ISSN 2251-7871

دوره 4

شماره 2 2014

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